To follow up on Christof Aegerter's seminar. last week indicating that there are challenges associated with detecting differences in tension across E-cadherin molecules in tissues cultured ex vivo, we are creating a wiki page called "FRET Discussion. Denise Montell has uploaded a detailed protocol for using the DE-CadTS in border cells as well as a description of the major image collection and processing challenges and solutions.” In general, if you are having problems reproducing someone else’s work, the best thing to do would be to contact them for more detailed protocols and information. This is both proper etiquette and also the best way to increase your probability of success.